Recombinant Human NR1D2 293 Cell Lysate NR1D2-3721HCL

Recombinant Human NR1D2 293 Cell Lysate


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Recombinant Human NR1D2 293 Cell Lysate

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Cat.No. : NR1D2-3721HCL
Description : Antigen standard for nuclear receptor subfamily 1, group D, member 2 (NR1D2), transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source : HEK 293 cells
Species : Human
Components : This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size : 0.1 mg
Storage Instruction : Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications : ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name : NR1D2 nuclear receptor subfamily 1, group D, member 2 [ Homo sapiens ]
Official Symbol : NR1D2
Synonyms : NR1D2; nuclear receptor subfamily 1, group D, member 2; nuclear receptor subfamily 1 group D member 2; BD73; EAR 1r; Hs.37288; HZF2; RVR; rev-erb-beta; rev-erba-alpha-related receptor; V-erbA-related protein 1-related; orphan nuclear hormone receptor BD73; nuclear receptor Rev-ErbA beta variant 1; nuclear receptor Rev-ErbA beta variant 2; EAR-1R;
Gene ID : 9975
mRNA Refseq : NM_001145425
Protein Refseq : NP_001138897
MIM : 602304
UniProt ID : Q14995
Chromosome Location : 3p24.1
Pathway : Diurnally regulated genes with circadian orthologs, organism-specific biosystem; Gene Expression, organism-specific biosystem; Generic Transcription Pathway, organism-specific biosystem; Nuclear Receptor transcription pathway, organism-specific biosystem; Nuclear Receptors, organism-specific biosystem;
Function : ligand-activated sequence-specific DNA binding RNA polymerase II transcription factor activity; metal ion binding; receptor activity; sequence-specific DNA binding; sequence-specific DNA binding transcription factor activity; steroid hormone receptor activity; zinc ion binding;

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