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Recombinant Human NR0B1 293 Cell Lysate

Cat.No.: NR0B1-3723HCL
Description: Antigen standard for nuclear receptor subfamily 0, group B, member 1 (NR0B1) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: NR0B1 nuclear receptor subfamily 0, group B, member 1 [ Homo sapiens ]
Official Symbol: NR0B1
Synonyms: NR0B1; nuclear receptor subfamily 0, group B, member 1; AHC, dosage sensitive sex reversal , DSS; nuclear receptor subfamily 0 group B member 1; AHCH; DAX1; nuclear receptor DAX1; nuclear receptor DAX-1; nuclear hormone receptor; DSS-AHC critical region on the X chromosome protein 1; AHC; AHX; DSS; GTD; HHG; DAX-1; NROB1; SRXY2;
Gene ID: 190
mRNA Refseq: NM_000475
Protein Refseq: NP_000466
MIM: 300473
UniProt ID: P51843
Chromosome Location: Xp21.3
Pathway: Androgen Receptor Signaling Pathway, organism-specific biosystem; Gene Expression, organism-specific biosystem; Generic Transcription Pathway, organism-specific biosystem; Nuclear Receptor transcription pathway, organism-specific biosystem; Nuclear Receptors, organism-specific biosystem; Regulation of Androgen receptor activity, organism-specific biosystem;
Function: AF-2 domain binding; DNA binding; DNA hairpin binding; RNA binding; double-stranded DNA binding; ligand-activated sequence-specific DNA binding RNA polymerase II transcription factor activity; ligand-activated sequence-specific DNA binding RNA polymerase II transcription factor activity; protein binding; protein domain specific binding; protein homodimerization activity; receptor activity; sequence-specific DNA binding; steroid hormone receptor activity; steroid hormone receptor binding; transcription corepressor activity; transcription factor binding;

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