"NPSR1" Related Products

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Recombinant Human NPSR1 293 Cell Lysate

Cat.No.: NPSR1-3728HCL
Description: Antigen standard for neuropeptide S receptor 1 (NPSR1), transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: NPSR1 neuropeptide S receptor 1 [ Homo sapiens ]
Official Symbol: NPSR1
Synonyms: NPSR1; neuropeptide S receptor 1; G protein coupled receptor 154 , GPR154; neuropeptide S receptor; GPRA; PGR14; G protein-coupled receptor 154; G-protein coupled receptor 154; G-protein coupled receptor PGR14; vasopressin receptor-related receptor 1; G protein-coupled receptor for asthma susceptibility; G-protein coupled receptor for asthma susceptibility; NPSR; VRR1; ASRT2; GPR154;
Gene ID: 387129
mRNA Refseq: NM_207172
Protein Refseq: NP_997055
MIM: 608595
UniProt ID: Q6W5P4
Chromosome Location: 7p14.3
Pathway: Class A/1 (Rhodopsin-like receptors), organism-specific biosystem; G alpha (q) signalling events, organism-specific biosystem; G alpha (s) signalling events, organism-specific biosystem; GPCR downstream signaling, organism-specific biosystem; GPCR ligand binding, organism-specific biosystem; Peptide ligand-binding receptors, organism-specific biosystem; Signal Transduction, organism-specific biosystem;
Function: G-protein coupled receptor activity; receptor activity; signal transducer activity; vasopressin receptor activity;

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