Recombinant Human NCOA3 293 Cell Lysate

• Cat.No.: NCOA3-3941HCL

Specification

• Description: Antigen standard for nuclear receptor coactivator 3 (NCOA3), transcript variant 2 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: NCOA3 nuclear receptor coactivator 3 [ Homo sapiens ]
• Official Symbol: NCOA3
• Synonyms: NCOA3; nuclear receptor coactivator 3; ACTR; AIB1; bHLHe42; CAGH16; KAT13B; p/CIP; RAC3; receptor associated coactivator 3; SRC 3; SRC3; thyroid hormone receptor activator molecule 1; TNRC16; TRAM 1; CBP-interacting protein; receptor-associated coactivator 3; amplified in breast cancer 1 protein; steroid receptor coactivator protein 3; class E basic helix-loop-helix protein 42; pCIP; AIB-1; CTG26; SRC-3; TNRC14; TRAM-1; MGC141848
• Gene ID: 8202
• mRNA Refseq: NM_001174087
• Protein Refseq: NP_001167558
• MIM: 601937
• UniProt ID: Q9Y6Q9
• Chromosome Location: 20q12
• Pathway: Androgen Receptor Signaling Pathway, organism-specific biosystem; BMAL1:CLOCK/NPAS2 Activates Circadian Expression, organism-specific biosystem; Circadian Clock, organism-specific biosystem; Circadian Repression of Expression by REV-ERBA, organism-specific biosystem; Developmental Biology, organism-specific biosystem; FOXA1 transcription factor network, organism-specific biosystem; Fatty acid, triacylglycerol, and ketone body metabolism, organism-specific biosystem
• Function: androgen receptor binding; chromatin binding; estrogen receptor binding; histone acetyltransferase activity; ligand-dependent nuclear receptor binding; ligand-dependent nuclear receptor transcription coactivator activity; nuclear hormone receptor binding; protein N-terminus binding; protein binding; signal transducer activity; thyroid hormone receptor binding; transcription coactivator activity; transcription coactivator activity; transcription coactivator activity; transferase activity, transferring acyl groups