"MPV17" Related Products

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Recombinant Human MPV17 293 Cell Lysate

Cat.No.: MPV17-4222HCL
Description: Antigen standard for MpV17 mitochondrial inner membrane protein (MPV17), nuclear gene encoding mitochondrial protein is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: MPV17 MpV17 mitochondrial inner membrane protein [ Homo sapiens ]
Official Symbol: MPV17
Synonyms: MPV17; MpV17 mitochondrial inner membrane protein; MpV17 transgene, murine homolog, glomerulosclerosis; protein Mpv17; glomerulosclerosis; SYM1; Mpv17, human homolog of glomerulosclerosis and nephrotic syndrome; MTDPS6;
Gene ID: 4358
mRNA Refseq: NM_002437
Protein Refseq: NP_002428
MIM: 137960
UniProt ID: P39210
Chromosome Location: 2p23.3
Pathway: Peroxisome, organism-specific biosystem; Peroxisome, conserved biosystem;
Function: molecular_function;

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