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Recombinant Human MOS 293 Cell Lysate

Cat.No.: MOS-4247HCL
Description: Antigen standard for v-mos Moloney murine sarcoma viral oncogene homolog (MOS) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: MOS v-mos Moloney murine sarcoma viral oncogene homolog [ Homo sapiens ]
Official Symbol: MOS
Synonyms: MOS; v-mos Moloney murine sarcoma viral oncogene homolog; proto-oncogene serine/threonine-protein kinase mos; c-mos; proto-oncogene c-Mos; oocyte maturation factor mos; oncogene MOS, Moloney murine sarcoma virus; MSV; MGC119962; MGC119963;
Gene ID: 4342
mRNA Refseq: NM_005372
Protein Refseq: NP_005363
MIM: 190060
UniProt ID: P00540
Chromosome Location: 8q11
Pathway: MAPK signaling pathway, organism-specific biosystem; MAPK signaling pathway, organism-specific biosystem; MAPK signaling pathway, conserved biosystem; Oocyte meiosis, organism-specific biosystem; Oocyte meiosis, conserved biosystem; Progesterone-mediated oocyte maturation, organism-specific biosystem; Progesterone-mediated oocyte maturation, conserved biosystem;
Function: ATP binding; nucleotide binding; protein serine/threonine kinase activity;

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