"MKNK1" Related Products

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Recombinant Human MKNK1 293 Cell Lysate

Cat.No.: MKNK1-4303HCL
Description: Antigen standard for MAP kinase interacting serine/threonine kinase 1 (MKNK1), transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: MKNK1 MAP kinase interacting serine/threonine kinase 1 [ Homo sapiens ]
Official Symbol: MKNK1
Synonyms: MKNK1; MAP kinase interacting serine/threonine kinase 1; MAP kinase-interacting serine/threonine-protein kinase 1; MNK1; MAPK signal-integrating kinase 1; MAP kinase signal-integrating kinase 1;
Gene ID: 8569
mRNA Refseq: NM_001135553
Protein Refseq: NP_001129025
MIM: 606724
UniProt ID: Q9BUB5
Chromosome Location: 1p33
Pathway: IL-2 Signaling Pathway, organism-specific biosystem; Insulin signaling pathway, organism-specific biosystem; Insulin signaling pathway, conserved biosystem; MAPK signaling pathway, organism-specific biosystem; MAPK signaling pathway, conserved biosystem; Negative regulation of FGFR signaling, organism-specific biosystem; Signal Transduction, organism-specific biosystem;
Function: ATP binding; ATP binding; nucleotide binding; protein binding; protein serine/threonine kinase activity; protein serine/threonine kinase activity;

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