"MGST3" Related Products

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Recombinant Human MGST3 293 Cell Lysate

Cat.No.: MGST3-4326HCL
Description: Antigen standard for microsomal glutathione S-transferase 3 (MGST3) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: MGST3 microsomal glutathione S-transferase 3 [ Homo sapiens ]
Official Symbol: MGST3
Synonyms: MGST3; microsomal glutathione S-transferase 3; GST III; microsomal glutathione S transferase III; microsomal GST 3; microsomal GST III; microsomal GST-3; microsomal GST-III; microsomal glutathione S-transferase III; GST-III;
Gene ID: 4259
mRNA Refseq: NM_004528
Protein Refseq: NP_004519
MIM: 604564
UniProt ID: O14880
Chromosome Location: 1q23
Pathway: Biological oxidations, organism-specific biosystem; Drug metabolism - cytochrome P450, organism-specific biosystem; Drug metabolism - cytochrome P450, conserved biosystem; Glutathione conjugation, organism-specific biosystem; Glutathione metabolism, organism-specific biosystem; Glutathione metabolism, conserved biosystem; Metabolism, organism-specific biosystem;
Function: glutathione peroxidase activity; glutathione transferase activity; peroxidase activity; transferase activity;

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