"MED27" Related Products


Recombinant Human MED27 293 Cell Lysate

Cat.No.: MED27-4385HCL
Description: Antigen standard for mediator complex subunit 27 (MED27) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: MED27 mediator complex subunit 27 [ Homo sapiens ]
Official Symbol: MED27
Synonyms: MED27; mediator complex subunit 27; cofactor required for Sp1 transcriptional activation, subunit 8, 34kDa , CRSP8; mediator of RNA polymerase II transcription subunit 27; CRSP34; TRAP37; CRSP complex subunit 8; p37 TRAP/SMCC/PC2 subunit; transcriptional coactivator CRSP34; cofactor required for Sp1 transcriptional activation, subunit 8, 34kDa; CRSP8; CRAP34; FLJ42238; MGC11274;
Gene ID: 9442
mRNA Refseq: NM_001253881
Protein Refseq: NP_001240810
MIM: 605044
UniProt ID: Q6P2C8
Chromosome Location: 9q34.13
Pathway: Developmental Biology, organism-specific biosystem; Fatty acid, triacylglycerol, and ketone body metabolism, organism-specific biosystem; Gene Expression, organism-specific biosystem; Generic Transcription Pathway, organism-specific biosystem; Metabolism, organism-specific biosystem; Metabolism of lipids and lipoproteins, organism-specific biosystem; PPARA Activates Gene Expression, organism-specific biosystem;
Function: transcription coactivator activity;

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