"MED20" Related Products

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Recombinant Human MED20 293 Cell Lysate

Cat.No.: MED20-4389HCL
Description: Antigen standard for mediator complex subunit 20 (MED20) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: MED20 mediator complex subunit 20 [ Homo sapiens ]
Official Symbol: MED20
Synonyms: MED20; mediator complex subunit 20; Trf (TATA binding protein related factor) proximal homolog (Drosophila) , TRFP; mediator of RNA polymerase II transcription subunit 20; DKFZp586D2223; PRO0213; hTRFP; TRF-proximal protein homolog; Trf (TATA binding protein-related factor)-proximal homolog; TRFP; MGC29869;
Gene ID: 9477
mRNA Refseq: NM_004275
Protein Refseq: NP_004266
MIM: 612915
UniProt ID: Q9H944
Chromosome Location: 6p21.1
Pathway: Developmental Biology, organism-specific biosystem; Fatty acid, triacylglycerol, and ketone body metabolism, organism-specific biosystem; Gene Expression, organism-specific biosystem; Generic Transcription Pathway, organism-specific biosystem; Metabolism, organism-specific biosystem; Metabolism of lipids and lipoproteins, organism-specific biosystem; PPARA Activates Gene Expression, organism-specific biosystem;
Function: DNA-directed RNA polymerase activity; RNA polymerase II transcription cofactor activity; protein binding;

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