"MDM4" Related Products

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Recombinant Human MDM4 293 Cell Lysate

Cat.No.: MDM4-4403HCL
Description: Antigen standard for Mdm4 p53 binding protein homolog (mouse) (MDM4), transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: MDM4 Mdm4 p53 binding protein homolog (mouse) [ Homo sapiens ]
Official Symbol: MDM4
Synonyms: MDM4; Mdm4 p53 binding protein homolog (mouse); Mdm4, transformed 3T3 cell double minute 4, p53 binding protein (mouse) , mouse double minute 4, human homolog of; p53 binding protein; protein Mdm4; MDMX; protein Mdmx; MDM4-related protein 1; mdm2-like p53-binding protein; double minute 4, human homolog of; p53-binding protein; Mdm4, transformed 3T3 cell double minute 4, p53 binding protein; HDMX; MRP1; MGC132766; DKFZp781B1423;
Gene ID: 4194
mRNA Refseq: NM_001204171
Protein Refseq: NP_001191100
MIM: 602704
UniProt ID: O15151
Chromosome Location: 1q32
Pathway: p53 pathway, organism-specific biosystem; p53 signaling pathway, organism-specific biosystem; p53 signaling pathway, conserved biosystem;
Function: enzyme binding; metal ion binding; protein binding; zinc ion binding; zinc ion binding;

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