"MDH2" Related Products

Expand
Expand
Expand

Recombinant Human MDH2 293 Cell Lysate

Cat.No.: MDH2-4407HCL
Description: Antigen standard for malate dehydrogenase 2, NAD (mitochondrial) (MDH2), nuclear gene encoding mitochondrial protein is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: MDH2 malate dehydrogenase 2, NAD (mitochondrial) [ Homo sapiens ]
Official Symbol: MDH2
Synonyms: MDH2; malate dehydrogenase 2, NAD (mitochondrial); malate dehydrogenase, mitochondrial; MDH; MOR1; M-MDH; MGC:3559;
Gene ID: 4191
mRNA Refseq: NM_005918
Protein Refseq: NP_005909
MIM: 154100
UniProt ID: P40926
Chromosome Location: 7cen-q22
Pathway: Citrate cycle (TCA cycle), organism-specific biosystem; Citrate cycle (TCA cycle), conserved biosystem; Citrate cycle, second carbon oxidation, 2-oxoglutarate =>oxaloacetate, organism-specific biosystem; Citrate cycle, second carbon oxidation, 2-oxoglutarate => oxaloacetate, conserved biosystem; Citric acid cycle (TCA cycle), organism-specific biosystem;
Function: L-malate dehydrogenase activity; L-malate dehydrogenase activity; L-malate dehydrogenase activity; malate dehydrogenase (NADP+) activity; nucleotide binding; oxidoreductase activity; protein self-association;

Online Inquiry

Note: There will be extra charge for optional service!

Please input "biomart" as verification code. Please review Creative BioMart's privacy policy for more information

Optional requirements on this protein    +Expand

Price Inquiry

Welcome! For price inquiries, please feel free to contact us through the form below. We will get back to you as soon as possible.