"MCM3" Related Products


Recombinant Human MCM3 293 Cell Lysate

Cat.No.: MCM3-4419HCL
Description: Antigen standard for minichromosome maintenance complex component 3 (MCM3) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: MCM3 minichromosome maintenance complex component 3 [ Homo sapiens ]
Official Symbol: MCM3
Synonyms: MCM3; minichromosome maintenance complex component 3; MCM3 minichromosome maintenance deficient 3 (S. cerevisiae) , minichromosome maintenance deficient (S. cerevisiae) 3; DNA replication licensing factor MCM3; p102; RLF subunit beta; hRlf beta subunit; DNA replication factor MCM3; cervical cancer proto-oncogene 5; minichromosome maintenance deficient 3; replication licensing factor, beta subunit; MCM3 minichromosome maintenance deficient 3; DNA polymerase alpha holoenzyme-associated protein P1; HCC5; P1.h; RLFB; P1-MCM3; MGC1157;
Gene ID: 4172
mRNA Refseq: NM_002388
Protein Refseq: NP_002379
MIM: 602693
UniProt ID: P25205
Chromosome Location: 6p12
Pathway: Activation of ATR in response to replication stress, organism-specific biosystem; Activation of the pre-replicative complex, organism-specific biosystem; Assembly of the pre-replicative complex, organism-specific biosystem; Cell Cycle, organism-specific biosystem; Cell Cycle Checkpoints, organism-specific biosystem; Cell Cycle, Mitotic, organism-specific biosystem; Cell cycle, organism-specific biosystem;
Function: ATP binding; DNA binding; helicase activity; hydrolase activity; nucleotide binding; protein binding;

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