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Recombinant Human MCM2 293 Cell Lysate

Cat.No.: MCM2-4420HCL
Description: Antigen standard for minichromosome maintenance complex component 2 (MCM2) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: MCM2 minichromosome maintenance complex component 2 [ Homo sapiens ]
Official Symbol: MCM2
Synonyms: MCM2; minichromosome maintenance complex component 2; CCNL1, CDCL1, MCM2 minichromosome maintenance deficient 2, mitotin (S. cerevisiae) , minichromosome maintenance deficient (S. cerevisiae) 2 (mitotin); DNA replication licensing factor MCM2; BM28; cdc19; D3S3194; KIAA0030; mitotin; cyclin-like 1; nuclear protein BM28; cell devision cycle-like 1; minichromosome maintenance protein 2 homolog; minichromosome maintenance deficient 2 (mitotin); MCM2 minichromosome maintenance deficient 2, mitotin; CCNL1; CDCL1; MITOTIN; MGC10606;
Gene ID: 4171
mRNA Refseq: NM_004526
Protein Refseq: NP_004517
MIM: 116945
UniProt ID: P49736
Chromosome Location: 3q21
Pathway: Activation of ATR in response to replication stress, organism-specific biosystem; Activation of the pre-replicative complex, organism-specific biosystem; Assembly of the pre-replicative complex, organism-specific biosystem; Cell Cycle, organism-specific biosystem; Cell Cycle Checkpoints, organism-specific biosystem; Cell Cycle, Mitotic, organism-specific biosystem; Cell cycle, organism-specific biosystem;
Function: ATP binding; DNA binding; DNA replication origin binding; helicase activity; histone binding; hydrolase activity; metal ion binding; nucleotide binding; protein binding;

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