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Recombinant Human MCCC1 293 Cell Lysate

Cat.No.: MCCC1-4429HCL
Description: Antigen standard for methylcrotonoyl-Coenzyme A carboxylase 1 (alpha) (MCCC1), nuclear gene encoding mitochondrial protein is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: MCCC1 methylcrotonoyl-CoA carboxylase 1 (alpha) [ Homo sapiens ]
Official Symbol: MCCC1
Synonyms: MCCC1; methylcrotonoyl-CoA carboxylase 1 (alpha); methylcrotonoyl Coenzyme A carboxylase 1 (alpha); methylcrotonoyl-CoA carboxylase subunit alpha, mitochondrial; MCCA; MCCase subunit alpha; 3-methylcrotonyl-CoA carboxylase 1; methylcrotonoyl-Coenzyme A carboxylase 1 (alpha); 3-methylcrotonyl-CoA:carbon dioxide ligase subunit alpha; 3-methylcrotonyl-CoA carboxylase biotin-containing subunit; MCC-B; FLJ25545; DKFZp686B20267;
Gene ID: 56922
mRNA Refseq: NM_020166
Protein Refseq: NP_064551
MIM: 609010
UniProt ID: Q96RQ3
Chromosome Location: 3q27.1
Pathway: Branched-chain amino acid catabolism, organism-specific biosystem; Leucine degradation, leucine =>acetoacetate + acetyl-CoA, organism-specific biosystem; Leucine degradation, leucine => acetoacetate + acetyl-CoA, conserved biosystem; Metabolic pathways, organism-specific biosystem; Metabolism, organism-specific biosystem;
Function: ATP binding; biotin binding; biotin carboxylase activity; ligase activity; metal ion binding; methylcrotonoyl-CoA carboxylase activity; nucleotide binding;

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