"MAPK4" Related Products

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Recombinant Human MAPK4 293 Cell Lysate

Cat.No.: MAPK4-4493HCL
Description: Antigen standard for mitogen-activated protein kinase 4 (MAPK4) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: MAPK4 mitogen-activated protein kinase 4 [ Homo sapiens ]
Official Symbol: MAPK4
Synonyms: MAPK4; mitogen-activated protein kinase 4; PRKM4; Erk3 related; Erk4; ERK-4; MAPK 4; p63-MAPK; Erk3-related; MAP kinase 4; MAP kinase isoform p63; extracellular signal-regulated kinase 4; ERK3; p63MAPK;
Gene ID: 5596
mRNA Refseq: NM_002747
Protein Refseq: NP_002738
MIM: 176949
UniProt ID: P31152
Chromosome Location: 18q21.2
Pathway: B Cell Receptor Signaling Pathway, organism-specific biosystem; Focal Adhesion, organism-specific biosystem; Insulin Signaling, organism-specific biosystem; Integrin-mediated cell adhesion, organism-specific biosystem; MAPK signaling pathway, organism-specific biosystem; MicroRNAs in cardiomyocyte hypertrophy, organism-specific biosystem; Regulation of Actin Cytoskeleton, organism-specific biosystem;
Function: ATP binding; MAP kinase activity; nucleotide binding; protein homodimerization activity; protein kinase binding; protein serine/threonine kinase activity;

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