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Recombinant Human MAP1LC3A 293 Cell Lysate
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Cat.No. : | MAP1LC3A-4514HCL |
Description : | Antigen standard for microtubule-associated protein 1 light chain 3 alpha (MAP1LC3A), transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection. |
Source : | HEK 293 cells |
Species : | Human |
Components : | This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol). |
Size : | 0.1 mg |
Storage Instruction : | Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment. |
Applications : | ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane. |
Gene Name : | MAP1LC3A microtubule-associated protein 1 light chain 3 alpha [ Homo sapiens ] |
Official Symbol : | MAP1LC3A |
Synonyms : | MAP1LC3A; microtubule-associated protein 1 light chain 3 alpha; ATG8E; LC3; LC3A; MAP1ALC3; MAP1BLC3; |
Gene ID : | 8199 |
mRNA Refseq : | NM_032514 |
Protein Refseq : | NP_115903 |
MIM : | 601242 |
UniProt ID : | Q9H492 |
Chromosome Location : | 20q11.22 |
◆ Recombinant Protein
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MAP1LC3A-2660R | Recombinant Rhesus monkey MAP1LC3A Protein, His-tagged | +Inquiry |
MAP1LC3A-301446H | Recombinant Human MAP1LC3A protein, GST-tagged | +Inquiry |
MAP1LC3A-9496M | Recombinant Mouse MAP1LC3A Protein | +Inquiry |
MAP1LC3A-297H | Recombinant Human MAP1LC3A protein, His/MBP-tagged | +Inquiry |
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MAP1LC3A MAP1LC3B MAP1LC3B2 Map2k1 MAP2K2 MAP2K3 MAP2K6 Map3k1 MAP3K14 MAP3K2 MAP3K3 MAP3K5 MAP3K8 MAP4K5 MAP2K4 MAP3K6 MAP2K5 MAP2K7 MAP3K11 MAP4K4 MAP4K2 MAP4K1 MAP1S Map2 MAP3K13 MAP3K15 MAP3K4 MAP3K7 MAP3K7IP1 MAP4 MAP3K10 MAP3K9 MAP1A MAP1B MAP7 MAP4K3 MAP3K12 MAP1LC3C MAP6D1 MAP7D1 MAP9 MAP6 MAP2K1IP1 MAP7D2 MAP7D3
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