"LPCAT1" Related Products

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Recombinant Human LPCAT1 293 Cell Lysate

Cat.No.: LPCAT1-4672HCL
Description: Antigen standard for lysophosphatidylcholine acyltransferase 1 (LPCAT1) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: LPCAT1 lysophosphatidylcholine acyltransferase 1 [ Homo sapiens ]
Official Symbol: LPCAT1
Synonyms: LPCAT1; lysophosphatidylcholine acyltransferase 1; acyltransferase like 2 , AYTL2; FLJ12443; LPCAT-1; lysoPAFAT; LPC acyltransferase 1; acyltransferase like 2; acyltransferase-like 2; lysoPC acyltransferase 1; lyso-PAF acetyltransferase; regulated by phosphonoformate; acetyl-CoA:lyso-PAF acetyltransferase; phosphonoformate immuno-associated protein 3; 1-acylglycerophosphocholine O-acyltransferase; 1-alkylglycerophosphocholine O-acetyltransferase; acyl-CoA:lysophosphatidylcholine acyltransferase 1; acetyl-CoA:lyso-platelet-activating factor acetyltransferase; AYTL2; lpcat; PFAAP3; FLJ41609;
Gene ID: 79888
mRNA Refseq: NM_024830
Protein Refseq: NP_079106
MIM: 610472
UniProt ID: Q8NF37
Chromosome Location: 5p15.33
Pathway: Ether lipid metabolism, organism-specific biosystem; Ether lipid metabolism, conserved biosystem; Fatty acid, triacylglycerol, and ketone body metabolism, organism-specific biosystem; Glycerophospholipid metabolism, organism-specific biosystem; Glycerophospholipid metabolism, conserved biosystem; Metabolic pathways, organism-specific biosystem; Metabolism, organism-specific biosystem;
Function: 1-acylglycerophosphocholine O-acyltransferase activity; 1-alkylglycerophosphocholine O-acetyltransferase activity; calcium ion binding; transferase activity, transferring acyl groups;

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