"EEF1DP1" Related Products

Recombinant Human LOC126037 293 Cell Lysate

Cat.No.: EEF1DP1-4702HCL
Description: Antigen standard for mo sapiens similar to Elongation factor 1-delta (EF-1-delta) (Antigen NY-CO-4), transcript variant 1 (LOC126037) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: EEF1DP1 eukaryotic translation elongation factor 1 delta pseudogene 1 [ Homo sapiens ]
Official Symbol: EEF1DP1
Gene ID: 126037
mRNA Refseq: XM_058967
Protein Refseq: XP_058967
Chromosome Location: 19p13.12
Pathway: Eukaryotic Translation Elongation, organism-specific biosystem; Metabolism of proteins, organism-specific biosystem; Translation, organism-specific biosystem

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