"LMO3" Related Products

Recombinant Human LMO3 293 Cell Lysate

Cat.No.: LMO3-4708HCL
Description: Antigen standard for LIM domain only 3 (rhombotin-like 2) (LMO3), transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: LMO3 LIM domain only 3 (rhombotin-like 2) [ Homo sapiens ]
Official Symbol: LMO3
Synonyms: LMO3; LIM domain only 3 (rhombotin-like 2); RBTNL2; LIM domain only protein 3; DAT1; Rhom 3; rhombotin-3; neuronal-specific transcription factor DAT1; RBTN3; RHOM3; Rhom-3; MGC26081;
Gene ID: 55885
mRNA Refseq: NM_018640
Protein Refseq: NP_061110
MIM: 180386
UniProt ID: Q8TAP4
Chromosome Location: 12p13
Function: metal ion binding; zinc ion binding;

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