||Recombinant Human Protein Kinase C alpha produced inSf9is a glycosylated, polypeptide chain containing amino acids 2-672 and having a molecular mass of 80 KD. This protein is the full-length form of the protein with an amino terminal poly His-tag. PKC- is purified by proprietary chromatographic techniques.
||Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells.
||Sf9 insect cells.
||TheUnit Definition is 7200-9000 units/mg. One unit of PKC-activity transfers 1 nmole of phosphate to a PKC substrate peptide (myelin basic protein 4-14) per minute at 30°C. Recombinant human active PKC-also phosphorylates full length myelin basic protein, histone, MARKS, and GSK-3. Activity requires incubation with lipid activators, and may vary depending on the substrate and reaction conditions.
||Greater than 95% as determined by SDS-PAGE.
||PKC- is supplied at a of 0.3mg/ml in 50mM Tris, pH 7.4, 0.1M NaCl, 20% glycerol, 1mM DTT, 0.1mM EDTA, 0.2mM PMSF and 0.03% Brij-35.
||Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. Avoid multiple freeze-thaw cycles.