"LIMS1" Related Products

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Recombinant Human LIMS1 293 Cell Lysate

Cat.No.: LIMS1-4736HCL
Description: Antigen standard for LIM and senescent cell antigen-like domains 1 (LIMS1) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: LIMS1 LIM and senescent cell antigen-like domains 1 [ Homo sapiens ]
Official Symbol: LIMS1
Synonyms: LIMS1; LIM and senescent cell antigen-like domains 1; LIM and senescent cell antigen-like-containing domain protein 1; PINCH; PINCH1; renal carcinoma antigen NY-REN-48; particularly interesting new Cys-His protein 1; PINCH-1;
Gene ID: 3987
mRNA Refseq: NM_001193482
Protein Refseq: NP_001180411
MIM: 602567
UniProt ID: P48059
Chromosome Location: 2q12.3
Pathway: Cell junction organization, organism-specific biosystem; Cell-Cell communication, organism-specific biosystem; Cell-extracellular matrix interactions, organism-specific biosystem; Regulation of cytoskeletal remodeling and cell spreading by IPP complex components, organism-specific biosystem;
Function: metal ion binding; protein binding; zinc ion binding;

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