"LDHC" Related Products

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Recombinant Human LDHC 293 Cell Lysate

Cat.No.: LDHC-4787HCL
Description: Antigen standard for lactate dehydrogenase C (LDHC), transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: LDHC lactate dehydrogenase C [ Homo sapiens ]
Official Symbol: LDHC
Synonyms: LDHC; lactate dehydrogenase C; L-lactate dehydrogenase C chain; cancer/testis antigen 32; CT32; LDH-C; LDH-X; LDH testis subunit; lactate dehydrogenase C4; lactate dehydrogenase c variant 1; lactate dehydrogenase c variant 3; lactate dehydrogenase c variant 4; LDH3; LDHX; MGC111073;
Gene ID: 3948
mRNA Refseq: NM_002301
Protein Refseq: NP_002292
MIM: 150150
UniProt ID: P07864
Chromosome Location: 11p15.1
Pathway: Cysteine and methionine metabolism, organism-specific biosystem; Cysteine and methionine metabolism, conserved biosystem; Glycolysis / Gluconeogenesis, organism-specific biosystem; Glycolysis / Gluconeogenesis, conserved biosystem; Glycolysis and Gluconeogenesis, organism-specific biosystem; Metabolic pathways, organism-specific biosystem; Propanoate metabolism, organism-specific biosystem;
Function: L-lactate dehydrogenase activity; nucleotide binding; oxidoreductase activity;

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