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Recombinant Human KRT6B 293 Cell Lysate

Cat.No. : KRT6B-4866HCL
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  • Gene Information
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Description : Antigen standard for keratin 6B (KRT6B) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source : HEK 293 cells
Species : Human
Components : This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size : 0.1 mg
Storage Instruction : Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications : ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name : KRT6B keratin 6B [ Homo sapiens ]
Official Symbol : KRT6B
Synonyms : KRT6B; keratin 6B; keratin like 1 (a type II keratin sequence) , KRTL1; keratin, type II cytoskeletal 6B; keratin-6B; cytokeratin 6B; cytokeratin-6B; type-II keratin Kb10; keratin, epidermal, type II, K6B; keratin-like 1 (a type II keratin sequence); K6B; PC2; CK6B; CK-6B; KRTL1;
Gene ID : 3854
mRNA Refseq : NM_005555
Protein Refseq : NP_005546
MIM : 148042
UniProt ID : P04259
Chromosome Location : 12q13.13
Function : structural constituent of cytoskeleton;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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