"KLF10" Related Products

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Recombinant Human KLF10 293 Cell Lysate

Cat.No.: KLF10-4933HCL
Description: Antigen standard for Kruppel-like factor 10 (KLF10), transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: KLF10 Kruppel-like factor 10 [ Homo sapiens ]
Official Symbol: KLF10
Synonyms: KLF10; Kruppel-like factor 10; TGFB inducible early growth response , TIEG; Krueppel-like factor 10; EGRA; TIEG1; TIEG-1; EGR-alpha; TGFB inducible early growth response; TGFB-inducible early growth response protein 1; transforming growth factor-beta-inducible early growth response protein 1; TIEG;
Gene ID: 7071
mRNA Refseq: NM_001032282
Protein Refseq: NP_001027453
MIM: 601878
UniProt ID: Q13118
Chromosome Location: 8q22.3
Function: DNA binding; metal ion binding; nucleic acid binding; protein binding; sequence-specific DNA binding transcription factor activity; zinc ion binding;

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