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Recombinant Human ILF2 293 Cell Lysate

Cat.No.: ILF2-5222HCL
Description: Antigen standard for interleukin enhancer binding factor 2, 45kDa (ILF2) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: ILF2 interleukin enhancer binding factor 2, 45kDa [ Homo sapiens ]
Official Symbol: ILF2
Synonyms: ILF2; interleukin enhancer binding factor 2, 45kDa; interleukin enhancer binding factor 2, 45kD; interleukin enhancer-binding factor 2; NF45; nuclear factor of activated T-cells 45 kDa; nuclear factor of activated T-cells, 45-kDa; PRO3063; MGC8391;
Gene ID: 3608
mRNA Refseq: NM_004515
Protein Refseq: NP_004506
MIM: 603181
UniProt ID: Q12905
Chromosome Location: 1q21.3
Function: ATP binding; DNA binding; double-stranded RNA binding; protein binding; transferase activity;

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