Recombinant Human IL29 293 Cell Lysate

• Cat.No.: IL29-5228HCL

Specification

• Description: Antigen standard for interleukin 29 (interferon, lambda 1) (IL29) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: IL29 interleukin 29 (interferon, lambda 1) [ Homo sapiens ]
• Official Symbol: IL29
• Synonyms: IL29; interleukin 29 (interferon, lambda 1); interleukin 29; interleukin-29; IFNL1; IL 29; IFN-lambda-1; cytokine Zcyto21; interferon lambda-1; interferon-lambda-1; interferon, lambda 1; IL-29
• Gene ID: 282618
• mRNA Refseq: NM_172140
• Protein Refseq: NP_742152
• MIM: 607403
• UniProt ID: Q8IU54
• Chromosome Location: 19q13.13
• Pathway: Cytokine-cytokine receptor interaction, organism-specific biosystem; Cytokine-cytokine receptor interaction, conserved biosystem; Jak-STAT signaling pathway, organism-specific biosystem; Jak-STAT signaling pathway, conserved biosystem
• Function: cytokine activity; interleukin-28 receptor binding; receptor binding

Reviews

10/12/2022

Storage conditions are very simple and can be stored for a long time.

04/30/2022

Anti-pollution ability is very strong, not easy to be polluted.

09/12/2019

Labeling effect is very good, can be fluorescent labeling and so on.

Q&As

How should the dose of IL29 be determined?

The dose of IL29 should be determined according to the specific disease and patient situation, and the most appropriate dose usually needs to be determined through clinical trials and individualized treatment.

What is the difference between IL29 and IL28?

IL29 and IL28 are two similar interferons that are similar in structure and function, but with some minor differences such as receptor affinity and signaling pathways.

What are the advantages of IL29 compared with traditional drugs?

IL29 is highly targeted and safe, and can stimulate the response of the own immune system, which has better therapeutic effect and fewer side effects than traditional drugs.

Does IL29 have antioxidant effects?

At present, there are no clear studies to prove that IL29 has a direct antioxidant effect, but it may indirectly affect oxidative stress response by regulating immune system function.

Recombinant IL29 has potential in the treatment of which autoimmune diseases?

IL29 has potential in the treatment of rheumatoid arthritis, hepatitis B, psoriasis and other autoimmune diseases.

Are there side effects of IL29?

It may cause some mild side effects, such as fever, fatigue, headache, etc., but it is generally reversible, and serious side effects are rare.