"IBTK" Related Products

Recombinant Human IBTK 293 Cell Lysate

Cat.No.: IBTK-5317HCL
Description: Antigen standard for inhibitor of Bruton agammaglobulinemia tyrosine kinase (IBTK) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: IBTK inhibitor of Bruton agammaglobulinemia tyrosine kinase [ Homo sapiens ]
Official Symbol: IBTK
Synonyms: IBTK; inhibitor of Bruton agammaglobulinemia tyrosine kinase; Bruton agammaglobulinemia tyrosine kinase inhibitor , BTKI; inhibitor of Bruton tyrosine kinase; DKFZP564B116; BTK-binding protein; inhibitor of Brutons tyrosine kinase; inhibitor of Brutons tyrosine kinase-beta; inhibitor of Brutons tyrosine kinase-alpha; inhibitor of Brutons tyrosine kinase-gamma; Bruton agammaglobulinemia tyrosine kinase inhibitor; BTKI; RP1-93K22.1; KIAA1417; MGC142256; MGC142258;
Gene ID: 25998
mRNA Refseq: NM_015525
Protein Refseq: NP_056340
MIM: 606457
UniProt ID: Q9P2D0
Chromosome Location: 6q14.3
Pathway: BCR signaling pathway, organism-specific biosystem;
Function: protein kinase binding; protein tyrosine kinase inhibitor activity;

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