"IARS" Related Products

Recombinant Human IARS 293 Cell Lysate

Cat.No.: IARS-5318HCL
Description: Antigen standard for isoleucyl-tRNA synthetase (IARS), transcript variant short is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: IARS isoleucyl-tRNA synthetase [ Homo sapiens ]
Official Symbol: IARS
Synonyms: IARS; isoleucyl-tRNA synthetase; isoleucine--tRNA ligase, cytoplasmic; IARS1; ILRS; isoleucine tRNA ligase 1; cytoplasmic; isoleucine tRNA ligase 1, cytoplasmic; isoleucyl-tRNA synthetase, cytoplasmic; IRS; ILERS; PRO0785; FLJ20736;
Gene ID: 3376
mRNA Refseq: NM_002161
Protein Refseq: NP_002152
MIM: 600709
UniProt ID: P41252
Chromosome Location: 9q21
Pathway: Aminoacyl-tRNA biosynthesis, organism-specific biosystem; Aminoacyl-tRNA biosynthesis, conserved biosystem; Aminoacyl-tRNA biosynthesis, eukaryotes, organism-specific biosystem; Aminoacyl-tRNA biosynthesis, eukaryotes, conserved biosystem; Cytosolic tRNA aminoacylation, organism-specific biosystem; Gene Expression, organism-specific biosystem; tRNA Aminoacylation, organism-specific biosystem;
Function: ATP binding; aminoacyl-tRNA ligase activity; isoleucine-tRNA ligase activity; isoleucine-tRNA ligase activity; ligase activity; nucleotide binding; protein binding;

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