"HLX" Related Products

Recombinant Human HLX 293 Cell Lysate

Cat.No.: HLX-5488HCL
Description: Antigen standard for H2.0-like homeobox (HLX) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: HLX H2.0-like homeobox [ Homo sapiens ]
Official Symbol: HLX
Synonyms: HLX; H2.0-like homeobox; H2.0 (Drosophila) like homeo box 1 , H2.0 like homeobox 1 , H2.0 like homeobox 1 (Drosophila) , HLX1; H2.0-like homeobox protein; HB24; H2.0-like homeobox 1; H2.0-like homeo box 1; H2.0-like homeo box-1; homeobox protein HB24; homeobox protein HLX1; HLX1;
Gene ID: 3142
mRNA Refseq: NM_021958
Protein Refseq: NP_068777
MIM: 142995
UniProt ID: Q14774
Chromosome Location: 1q41
Pathway: IL12-mediated signaling events, organism-specific biosystem;
Function: sequence-specific DNA binding; transcription regulatory region sequence-specific DNA binding;

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