"HCLS1" Related Products

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Recombinant Human HCLS1 293 Cell Lysate

Cat.No.: HCLS1-5611HCL
Description: Antigen standard for hematopoietic cell-specific Lyn substrate 1 (HCLS1) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: HCLS1 hematopoietic cell-specific Lyn substrate 1 [ Homo sapiens ]
Official Symbol: HCLS1
Synonyms: HCLS1; hematopoietic cell-specific Lyn substrate 1; hematopoietic lineage cell-specific protein; cortactin like; CTTNL; HS1; p75; lckBP1;
Gene ID: 3059
mRNA Refseq: NM_005335
Protein Refseq: NP_005326
MIM: 601306
UniProt ID: P14317
Chromosome Location: 3q13
Pathway: B Cell Receptor Signaling Pathway, organism-specific biosystem; Bacterial invasion of epithelial cells, organism-specific biosystem; Bacterial invasion of epithelial cells, conserved biosystem; Fc-epsilon receptor I signaling in mast cells, organism-specific biosystem; IL-5 Signaling Pathway, organism-specific biosystem; Pathogenic Escherichia coli infection, organism-specific biosystem; Pathogenic Escherichia coli infection, conserved biosystem;
Function: DNA binding; SH3 domain binding; protein complex binding; sequence-specific DNA binding transcription factor activity;

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