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Active Recombinant Human Protein Tyrosine Phosphatase, Non-receptor Type 6

Cat.No. : PTPN6-71H
Product Overview : Recombinant Human PTPN6 produced inE.Coliis a single,non-glycosylated polypeptide chain conjtaining 300 amino acids and having a molecular mass of 34.3 kDa. The protein coding region of the catalytic domain of SHP-1 (amino acids 243-541). The recombinant SHP-1 protein was purified by FPLC gel-filtration chromatography, after refolding of the isolated inclusion bodies in a redox buffer. Additional amino acid(Met) is attached at N-terminus.
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Cat. No. : PTPN6-71H
Description : SHP-1 is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. N-terminal part of this PTP contains two tandem Src homolog (SH2) domains, which act as protein phospho-tyrosine binding domains, and mediate the interaction of this PTP with its substrates. This PTP is expressed primarily in hematopoietic cells, and functions as an important regulator of multiple signaling pathways in hematopoietic cells. This PTP has been shown to interact with, and dephosphorylate a wide spectrum of phospho-proteins involved in hematopoietic cell signaling. Multiple alternatively spliced variants of this gene, which encode distinct isoforms, have been reported.
Source : Escherichia Coli
Amino Acid Sequence : MGFWEEFESLQKQEVKNLHQRLEGQ RPENKGKNRYKNILPFDHSRVILQG RDSNIPGSDYINANYIKNQLLGPDE NAKTYIASQGCLEATVNDFWQMAWQ ENSRVIVMTTREVEKGRNKCVPYWP EVGMQRAYGPYSVTNCGEHDTTEYK LRTLQVSPLDNGDLIREIWHYQYLS WPDHGVPSEPGGVLSFLDQINQRQE SLPHAGPIIVHCSAGIGRTGTIIVI DMLMENISTKGLDCDIDIQKTIQMV RAQRSGMVQTEAQYKFIYVAIAQFI ETTKKKLEVLQSQKGQESEYGNITY
Physical Appearance : Sterile filtered colorless solution.
Purity : Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Formulation : The protein (1mg/ml) contains 25mM Tris-HCl, pH 7.5, 2mM β-mercaptoethanol, 1mM EDTA, 1mMDTT and 20%Glycerol.
Unit Defenition : One unit will hydrolyze 1nanomole of p-nitrophenylphosphatate per minute at pH 7.5 at 37°C using 10mM of substrate.
Activity : 5,000 U/mg
Storage : Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Publication :
Role of PPM1A and anti-PPM1A Autoantibodies in Ankylosing Spondylitis. (2014)
Role of Protein Phosphatase Magnesium-Dependent 1A and Anti–Protein Phosphatase Magnesium-Dependent 1A Autoantibodies in Ankylosing Spondylitis (2014)
Gene Name : PTPN6 protein tyrosine phosphatase, non-receptor type 6 [ Homo sapiens ]
Synonyms : HCP; HCPH; SHP1; SHP-1; HPTP1C; PTP-1C; SHP-1L; SH-PTP1; PTPN6; protein tyrosine phosphatase, non-receptor type 6; SHP1; hematopoietic cell phosphatase; protein-tyrosine phosphatase 1C; hematopoietic cell protein-tyrosine phosphatase; EC 3.1.3.48;Hematopoietic cell protein-tyrosine phosphatase; Protein-tyrosine phosphatase SHP-1
Gene ID : 5777
mRNA Refseq : NM_002831
Protein Refseq : NP_002822
MIM : 176883
UniProt ID : P29350
Chromosome Location : 12p13.31
Pathway : Adherens junction; B cell receptor signaling pathway; Jak-STAT signaling pathway; Natural killer cell mediated cytotoxicity; T cell receptor signaling pathway; Hemostasis; Signaling in Immune system
Function : hydrolase activity; protein binding; protein tyrosine phosphatase activity

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (5)

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How does PTPN6 function as a negative regulator in immune cells? 09/13/2022

PTPN6 acts as a phosphatase, removing phosphate groups from specific tyrosine residues in signaling molecules, thereby downregulating immune cell activation.

Can PTPN6 be a potential target for immunotherapy? 08/04/2020

Yes, PTPN6 modulation is being explored as a potential target for immunotherapies aimed at enhancing or suppressing immune responses based on the clinical context.

How does PTPN6 impact T cell signaling, and why is this important in clinical contexts? 09/09/2018

PTPN6 negatively regulates T cell receptor signaling, and its modulation can have implications in controlling excessive immune responses seen in autoimmune diseases.

Are there specific diseases where the clinical application of PTPN6 is being investigated? 10/02/2017

Yes, PTPN6 is being studied in diseases like rheumatoid arthritis, lupus, and certain types of leukemia and lymphoma.

In what ways can PTPN6 be targeted for therapeutic interventions? 08/24/2016

Researchers are exploring small molecule inhibitors and gene therapies to modulate PTPN6 activity, with potential applications in autoimmune disorders and cancer treatment.

Customer Reviews (3)

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Reviews
07/01/2020

    Structural biology researchers can leverage its properties to better understand the three-dimensional architecture and conformational changes of proteins, gaining valuable insights into their function and interactions.

    04/03/2018

      PTPN6 protein is highly recommended for various research applications, including ELISA and protein electron microscopy structure analysis.

      12/07/2017

        PTPN6 protein might have biomarker properties, meaning its levels or activity could be indicative of disease progression or treatment response.

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