Active Recombinant Human Protein Tyrosine Phosphatase, Non-receptor Type 6
Cat.No. : | PTPN6-71H |
Product Overview : | Recombinant Human PTPN6 produced inE.Coliis a single,non-glycosylated polypeptide chain conjtaining 300 amino acids and having a molecular mass of 34.3 kDa. The protein coding region of the catalytic domain of SHP-1 (amino acids 243-541). The recombinant SHP-1 protein was purified by FPLC gel-filtration chromatography, after refolding of the isolated inclusion bodies in a redox buffer. Additional amino acid(Met) is attached at N-terminus. |
- Specification
- Gene Information
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Cat. No. : | PTPN6-71H |
Description : | SHP-1 is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. N-terminal part of this PTP contains two tandem Src homolog (SH2) domains, which act as protein phospho-tyrosine binding domains, and mediate the interaction of this PTP with its substrates. This PTP is expressed primarily in hematopoietic cells, and functions as an important regulator of multiple signaling pathways in hematopoietic cells. This PTP has been shown to interact with, and dephosphorylate a wide spectrum of phospho-proteins involved in hematopoietic cell signaling. Multiple alternatively spliced variants of this gene, which encode distinct isoforms, have been reported. |
Source : | Escherichia Coli |
Amino Acid Sequence : | MGFWEEFESLQKQEVKNLHQRLEGQ RPENKGKNRYKNILPFDHSRVILQG RDSNIPGSDYINANYIKNQLLGPDE NAKTYIASQGCLEATVNDFWQMAWQ ENSRVIVMTTREVEKGRNKCVPYWP EVGMQRAYGPYSVTNCGEHDTTEYK LRTLQVSPLDNGDLIREIWHYQYLS WPDHGVPSEPGGVLSFLDQINQRQE SLPHAGPIIVHCSAGIGRTGTIIVI DMLMENISTKGLDCDIDIQKTIQMV RAQRSGMVQTEAQYKFIYVAIAQFI ETTKKKLEVLQSQKGQESEYGNITY |
Physical Appearance : | Sterile filtered colorless solution. |
Purity : | Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE. |
Formulation : | The protein (1mg/ml) contains 25mM Tris-HCl, pH 7.5, 2mM β-mercaptoethanol, 1mM EDTA, 1mMDTT and 20%Glycerol. |
Unit Defenition : | One unit will hydrolyze 1nanomole of p-nitrophenylphosphatate per minute at pH 7.5 at 37°C using 10mM of substrate. |
Activity : | 5,000 U/mg |
Storage : | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles. |
Publication : |
Role of PPM1A and anti-PPM1A Autoantibodies in Ankylosing Spondylitis. (2014)
Role of Protein Phosphatase Magnesium-Dependent 1A and Anti–Protein Phosphatase Magnesium-Dependent 1A Autoantibodies in Ankylosing Spondylitis (2014)
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Gene Name : | PTPN6 protein tyrosine phosphatase, non-receptor type 6 [ Homo sapiens ] |
Synonyms : | HCP; HCPH; SHP1; SHP-1; HPTP1C; PTP-1C; SHP-1L; SH-PTP1; PTPN6; protein tyrosine phosphatase, non-receptor type 6; SHP1; hematopoietic cell phosphatase; protein-tyrosine phosphatase 1C; hematopoietic cell protein-tyrosine phosphatase; EC 3.1.3.48;Hematopoietic cell protein-tyrosine phosphatase; Protein-tyrosine phosphatase SHP-1 |
Gene ID : | 5777 |
mRNA Refseq : | NM_002831 |
Protein Refseq : | NP_002822 |
MIM : | 176883 |
UniProt ID : | P29350 |
Chromosome Location : | 12p13.31 |
Pathway : | Adherens junction; B cell receptor signaling pathway; Jak-STAT signaling pathway; Natural killer cell mediated cytotoxicity; T cell receptor signaling pathway; Hemostasis; Signaling in Immune system |
Function : | hydrolase activity; protein binding; protein tyrosine phosphatase activity |
Products Types
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Ptpn6-1102M | Recombinant Mouse Ptpn6 Protein, MYC/DDK-tagged | +Inquiry |
PTPN6-0697H | Recombinant Human PTPN6 Protein (M1-K595), Tag Free | +Inquiry |
PTPN6-3129H | Recombinant Human PTPN6 Protein, MYC/DDK-tagged | +Inquiry |
PTPN6-0698H | Recombinant Human PTPN6 Protein (M1-K595), His/Strep tagged | +Inquiry |
◆ Lysates | ||
PTPN6-001MCL | Recombinant Mouse PTPN6 cell lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (5)
Ask a questionPTPN6 acts as a phosphatase, removing phosphate groups from specific tyrosine residues in signaling molecules, thereby downregulating immune cell activation.
Yes, PTPN6 modulation is being explored as a potential target for immunotherapies aimed at enhancing or suppressing immune responses based on the clinical context.
PTPN6 negatively regulates T cell receptor signaling, and its modulation can have implications in controlling excessive immune responses seen in autoimmune diseases.
Yes, PTPN6 is being studied in diseases like rheumatoid arthritis, lupus, and certain types of leukemia and lymphoma.
Researchers are exploring small molecule inhibitors and gene therapies to modulate PTPN6 activity, with potential applications in autoimmune disorders and cancer treatment.
Customer Reviews (3)
Write a reviewStructural biology researchers can leverage its properties to better understand the three-dimensional architecture and conformational changes of proteins, gaining valuable insights into their function and interactions.
PTPN6 protein is highly recommended for various research applications, including ELISA and protein electron microscopy structure analysis.
PTPN6 protein might have biomarker properties, meaning its levels or activity could be indicative of disease progression or treatment response.
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