"GYS1" Related Products

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Recombinant Human GYS1 293 Cell Lysate

Cat.No.: GYS1-5670HCL
Description: Antigen standard for glycogen synthase 1 (muscle) (GYS1), transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: GYS1 glycogen synthase 1 (muscle) [ Homo sapiens ]
Official Symbol: GYS1
Synonyms: GYS1; glycogen synthase 1 (muscle); GYS; glycogen [starch] synthase, muscle; GSY;
Gene ID: 2997
mRNA Refseq: NM_001161587
Protein Refseq: NP_001155059
MIM: 138570
UniProt ID: P13807
Chromosome Location: 19q13.3
Pathway: Glucose metabolism, organism-specific biosystem; Glycogen Metabolism, organism-specific biosystem; Glycogen synthesis, organism-specific biosystem; Insulin Signaling, organism-specific biosystem; Insulin signaling pathway, organism-specific biosystem; Insulin signaling pathway, conserved biosystem; Insulin-mediated glucose transport, organism-specific biosystem;
Function: glucose binding; glycogen (starch) synthase activity; glycogen (starch) synthase activity; protein binding; protein kinase binding; transferase activity, transferring glycosyl groups;

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