Recombinant Human GRIP1 293 Cell Lysate

• Cat.No.: GRIP1-5741HCL

Specification

• Description: Antigen standard for glutamate receptor interacting protein 1 (GRIP1) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: GRIP1 glutamate receptor interacting protein 1 [ Homo sapiens ]
• Official Symbol: GRIP1
• Synonyms: GRIP1; glutamate receptor interacting protein 1; glutamate receptor-interacting protein 1; GRIP
• Gene ID: 23426
• mRNA Refseq: NM_001178074
• Protein Refseq: NP_001171545
• MIM: 604597
• UniProt ID: Q9Y3R0
• Chromosome Location: 12q13.13
• Pathway: Glutamate Binding, Activation of AMPA Receptors and Synaptic Plasticity, organism-specific biosystem; Neuronal System, organism-specific biosystem; Neurotransmitter Receptor Binding And Downstream Transmission In ThePostsynaptic Cell, organism-specific biosystem; Trafficking of AMPA receptors, organism-specific biosystem; Trafficking of GluR2-containing AMPA receptors, organism-specific biosystem; Transmission across Chemical Synapses, organism-specific biosystem
• Function: androgen receptor binding; beta-catenin binding; protein C-terminus binding; receptor signaling complex scaffold activity; transcription coactivator activity