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Recombinant Human GRIK2 293 Cell Lysate

Cat.No. : GRIK2-5747HCL
  • Specification
  • Gene Information
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Description : Antigen standard for glutamate receptor, ionotropic, kainate 2 (GRIK2), transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source : HEK 293 cells
Species : Human
Components : This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size : 0.1 mg
Storage Instruction : Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications : ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name : GRIK2 glutamate receptor, ionotropic, kainate 2 [ Homo sapiens ]
Official Symbol : GRIK2
Synonyms : GRIK2; glutamate receptor, ionotropic, kainate 2; GLUR6; glutamate receptor, ionotropic kainate 2; GluK2; MRT6; gluR-6; bA487F5.1; glutamate receptor 6; glutamate receptor form A; glutamate receptor form B; glutamate receptor form C; glutamate receptor form D; glutamate receptor form E; excitatory amino acid receptor 4; EAA4; GLR6; GLUK6; MGC74427;
Gene ID : 2898
mRNA Refseq : NM_001166247
Protein Refseq : NP_001159719
MIM : 138244
UniProt ID : Q13002
Chromosome Location : 6q16.3
Pathway : Activation of Ca-permeable Kainate Receptor, organism-specific biosystem; Activation of Kainate Receptors upon glutamate binding, organism-specific biosystem; Activation of Na-permeable Kainate Receptors, organism-specific biosystem; Glutamatergic synapse, organism-specific biosystem; Glutamatergic synapse, conserved biosystem; Ionotropic activity of Kainate Receptors, organism-specific biosystem; Neuroactive ligand-receptor interaction, organism-specific biosystem;
Function : PDZ domain binding; extracellular-glutamate-gated ion channel activity; ion channel activity; kainate selective glutamate receptor activity; protein homodimerization activity; receptor activity; ubiquitin conjugating enzyme binding; ubiquitin protein ligase binding;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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