Recombinant Human GOPC 293 Cell Lysate GOPC-5831HCL

Recombinant Human GOPC 293 Cell Lysate


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Recombinant Human GOPC 293 Cell Lysate

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Cat.No. : GOPC-5831HCL
Description : Antigen standard for golgi-associated PDZ and coiled-coil motif containing (GOPC), transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source : HEK 293 cells
Species : Human
Components : This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size : 0.1 mg
Storage Instruction : Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications : ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name : GOPC golgi-associated PDZ and coiled-coil motif containing [ Homo sapiens ]
Official Symbol : GOPC
Synonyms : GOPC; golgi-associated PDZ and coiled-coil motif containing; Golgi-associated PDZ and coiled-coil motif-containing protein; CAL; dJ94G16.2; FIG; GOPC1; PIST; dJ94G16.2 PIST; fused in glioblastoma; CFTR-associated ligand; PDZ protein interacting specifically with TC10; Golgi associated PDZ and coiled-coil motif containing protein; PDZ/coiled-coil domain binding partner for the rho-family GTPase TC10;
Gene ID : 57120
mRNA Refseq : NM_001017408
Protein Refseq : NP_001017408
MIM : 606845
UniProt ID : Q9HD26
Chromosome Location : 6q21
Function : cystic fibrosis transmembrane conductance regulator binding; frizzled binding; protein C-terminus binding; protein binding; protein homodimerization activity; small GTPase regulator activity;

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