Recombinant Human GNAS 293 Cell Lysate

• Cat.No.: GNAS-5866HCL

Specification

• Description: Antigen standard for GNAS complex locus (GNAS), transcript variant 4 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: GNAS GNAS complex locus [ Homo sapiens ]
• Official Symbol: GNAS
• Synonyms: GNAS; GNAS complex locus; GNAS1, guanine nucleotide binding protein (G protein), alpha stimulating activity polypeptide 1; protein ALEX; GNASXL; GPSA; NESP; NESP55; SCG6; secretogranin VI; extra large alphas protein; neuroendocrine secretory protein; guanine nucleotide regulatory protein; alternative gene product encoded by XL-exon; adenylate cyclase-stimulating G alpha protein; guanine nucleotide-binding protein G(s) subunit alpha isoforms XLas; guanine nucleotide binding protein (G protein), alpha stimulating activity polypeptide 1; AHO; GSA; GSP; POH; GNAS1; PHP1A; PHP1B; PHP1C; C20orf45; MGC33735
• Gene ID: 2778
• mRNA Refseq: NM_000516
• Protein Refseq: NP_000507
• UniProt ID: O95467
• Chromosome Location: 20q13.2-q13.3
• Pathway: Amoebiasis, organism-specific biosystem; Amoebiasis, conserved biosystem; Amphetamine addiction, organism-specific biosystem; Amphetamine addiction, conserved biosystem; Aquaporin-mediated transport, organism-specific biosystem; Bile secretion, organism-specific biosystem; Bile secretion, conserved biosystem
• Function: G-protein beta/gamma-subunit complex binding; GTP binding; GTPase activity; GTPase activity; adenylate cyclase activity; guanyl nucleotide binding; guanyl-nucleotide exchange factor activity; ionotropic glutamate receptor binding; metal ion binding; molecular_function; mu-type opioid receptor binding; nucleotide binding; signal transducer activity; signal transducer activity