Recombinant Human GNAI3 293 Cell Lysate

• Cat.No.: GNAI3-5869HCL

Specification

• Description: Antigen standard for guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 3 (GNAI3) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: GNAI3 guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 3 [ Homo sapiens ]
• Official Symbol: GNAI3
• Synonyms: GNAI3; guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 3; guanine nucleotide-binding protein G(k) subunit alpha; 87U6; g(i) alpha-3; FLJ26559
• Gene ID: 2773
• mRNA Refseq: NM_006496
• Protein Refseq: NP_006487
• MIM: 139370
• UniProt ID: P08754
• Chromosome Location: 1p13
• Pathway: ADP signalling through P2Y purinoceptor 12, organism-specific biosystem; Activation of GABAB receptors, organism-specific biosystem; Adenylate cyclase inhibitory pathway, organism-specific biosystem; Axon guidance, organism-specific biosystem; Axon guidance, conserved biosystem; CXCR3-mediated signaling events, organism-specific biosystem; Calcium Regulation in the Cardiac Cell, organism-specific biosystem
• Function: G-protein beta/gamma-subunit complex binding; G-protein coupled serotonin receptor binding; GTP binding; GTPase activity; GTPase activity; guanyl nucleotide binding; guanyl-nucleotide exchange factor activity; metal ion binding; nucleotide binding; phosphatidylinositol phospholipase C activity; signal transducer activity