Recombinant Human GNA15 293 Cell Lysate

• Cat.No.: GNA15-5871HCL

Specification

• Description: Antigen standard for guanine nucleotide binding protein (G protein), alpha 15 (Gq class) (GNA15) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: GNA15 guanine nucleotide binding protein (G protein), alpha 15 (Gq class) [ Homo sapiens ]
• Official Symbol: GNA15
• Synonyms: GNA15; guanine nucleotide binding protein (G protein), alpha 15 (Gq class); guanine nucleotide-binding protein subunit alpha-15; GNA16; g alpha-15; g alpha-16; G-protein subunit alpha-15; G-protein subunit alpha-16; guanine nucleotide-binding protein subunit alpha-16
• Gene ID: 2769
• mRNA Refseq: NM_002068
• Protein Refseq: NP_002059
• MIM: 139314
• UniProt ID: P30679
• Chromosome Location: 19p13.3
• Pathway: ADP signalling through P2Y purinoceptor 1, organism-specific biosystem; Amoebiasis, organism-specific biosystem; Amoebiasis, conserved biosystem; Arf6 signaling events, organism-specific biosystem; Calcium signaling pathway, organism-specific biosystem; Calcium signaling pathway, conserved biosystem; Chagas disease (American trypanosomiasis), organism-specific biosystem
• Function: G-protein beta/gamma-subunit complex binding; G-protein coupled receptor binding; GTP binding; GTPase activity; guanyl nucleotide binding; guanyl-nucleotide exchange factor activity; metal ion binding; nucleotide binding; phospholipase C activity; signal transducer activity