"GFI1" Related Products

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Recombinant Human GFI1 293 Cell Lysate

Cat.No.: GFI1-5953HCL
Description: Antigen standard for growth factor independent 1 transcription repressor (GFI1), transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: GFI1 growth factor independent 1 transcription repressor [ Homo sapiens ]
Official Symbol: GFI1
Synonyms: GFI1; growth factor independent 1 transcription repressor; growth factor independent 1 , ZNF163; zinc finger protein Gfi-1; zinc finger protein 163; growth factor independence-1; growth factor independent protein 1; SCN2; GFI-1; ZNF163; FLJ94509;
Gene ID: 2672
mRNA Refseq: NM_001127215
Protein Refseq: NP_001120687
MIM: 600871
UniProt ID: Q99684
Chromosome Location: 1p22
Pathway: Validated targets of C-MYC transcriptional repression, organism-specific biosystem;
Function: DNA binding; metal ion binding; protein binding; transcription regulatory region DNA binding; zinc ion binding;

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