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Recombinant Human GCLM 293 Cell Lysate

Cat.No.: GCLM-5982HCL
Description: Antigen standard for glutamate-cysteine ligase, modifier subunit (GCLM) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: GCLM glutamate-cysteine ligase, modifier subunit [ Homo sapiens ]
Official Symbol: GCLM
Synonyms: GCLM; glutamate-cysteine ligase, modifier subunit; GLCLR; glutamate--cysteine ligase regulatory subunit; gamma glutamylcysteine synthetase; GCS light chain; GSC light chain; gamma-ECS regulatory subunit; glutamate--cysteine ligase modifier subunit; glutamate-cysteine ligase regulatory protein; gamma-glutamylcysteine synthetase regulatory subunit; glutamate-cysteine ligase (gamma-glutamylcysteine synthetase), regulatory (30.8kD);
Gene ID: 2730
mRNA Refseq: NM_002061
Protein Refseq: NP_002052
MIM: 601176
UniProt ID: P48507
Chromosome Location: 1p21
Pathway: Biological oxidations, organism-specific biosystem; Glutathione biosynthesis, glutamate =>glutathione, organism-specific biosystem; Glutathione biosynthesis, glutamate => glutathione, conserved biosystem; Glutathione conjugation, organism-specific biosystem; Glutathione metabolism, organism-specific biosystem;
Function: contributes_to glutamate-cysteine ligase activity; contributes_to glutamate-cysteine ligase activity; glutamate-cysteine ligase catalytic subunit binding; protein heterodimerization activity;

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