Recombinant Human GCLC 293 Cell Lysate

• Cat.No.: GCLC-5983HCL

Specification

• Description: Antigen standard for glutamate-cysteine ligase, catalytic subunit (GCLC) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: GCLC glutamate-cysteine ligase, catalytic subunit [ Homo sapiens ]
• Official Symbol: GCLC
• Synonyms: GCLC; glutamate-cysteine ligase, catalytic subunit; GLCL, GLCLC; glutamate--cysteine ligase catalytic subunit; GCS; gamma-ECS; GCS heavy chain; gamma-glutamylcysteine synthetase; GCL; GLCL; GLCLC
• Gene ID: 2729
• mRNA Refseq: NM_001197115
• Protein Refseq: NP_001184044
• MIM: 606857
• UniProt ID: P48506
• Chromosome Location: 6p12
• Pathway: Biological oxidations, organism-specific biosystem; Glutathione biosynthesis, glutamate =>glutathione, organism-specific biosystem; Glutathione biosynthesis, glutamate => glutathione, conserved biosystem; Glutathione conjugation, organism-specific biosystem; Glutathione metabolism, organism-specific biosystem
• Function: ADP binding; ATP binding; coenzyme binding; glutamate binding; glutamate-cysteine ligase activity; glutamate-cysteine ligase activity; ligase activity; magnesium ion binding; nucleotide binding; protein heterodimerization activity