"GCAT" Related Products

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Recombinant Human GCAT 293 Cell Lysate

Cat.No.: GCAT-5993HCL
Description: Antigen standard for glycine C-acetyltransferase (GCAT), nuclear gene encoding mitochondrial protein, transcript variant 2 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: GCAT glycine C-acetyltransferase [ Homo sapiens ]
Official Symbol: GCAT
Synonyms: GCAT; glycine C-acetyltransferase; glycine C acetyltransferase (2 amino 3 ketobutyrate coenzyme A ligase); 2-amino-3-ketobutyrate coenzyme A ligase, mitochondrial; 2 amino 3 ketobutyrate coenzyme A ligase; KBL; AKB ligase; aminoacetone synthase; glycine acetyltransferase; 2-amino-3-ketobutyrate-CoA ligase; MGC23053;
Gene ID: 23464
mRNA Refseq: NM_001171690
Protein Refseq: NP_001165161
MIM: 607422
UniProt ID: O75600
Chromosome Location: 22q13.1
Pathway: Glycine, serine and threonine metabolism, organism-specific biosystem; Glycine, serine and threonine metabolism, conserved biosystem; threonine degradation II, organism-specific biosystem;
Function: glycine C-acetyltransferase activity; pyridoxal phosphate binding; transferase activity, transferring acyl groups; transferase activity, transferring nitrogenous groups;

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