"FDFT1" Related Products


Recombinant Human FDFT1 293 Cell Lysate

Cat.No.: FDFT1-6271HCL
Description: Antigen standard for farnesyl-diphosphate farnesyltransferase 1 (FDFT1) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: FDFT1 farnesyl-diphosphate farnesyltransferase 1 [ Homo sapiens ]
Official Symbol: FDFT1
Synonyms: FDFT1; farnesyl-diphosphate farnesyltransferase 1; squalene synthase; squalene synthetase; FPP:FPP farnesyltransferase; presqualene-di-diphosphate synthase; SS; SQS; DGPT; ERG9;
Gene ID: 2222
mRNA Refseq: NM_004462
Protein Refseq: NP_004453
MIM: 184420
UniProt ID: P37268
Chromosome Location: 8p23.1-p22
Pathway: Cholesterol Biosynthesis, organism-specific biosystem; Cholesterol biosynthesis, organism-specific biosystem; Fatty acid, triacylglycerol, and ketone body metabolism, organism-specific biosystem; Metabolic pathways, organism-specific biosystem; Metabolism, organism-specific biosystem; Metabolism of lipids and lipoproteins, organism-specific biosystem; PPARA Activates Gene Expression, organism-specific biosystem;
Function: farnesyl-diphosphate farnesyltransferase activity; oxidoreductase activity; squalene synthase activity; transferase activity, transferring alkyl or aryl (other than methyl) groups;

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