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Recombinant Human FASTK 293 Cell Lysate

Cat.No. : FASTK-6322HCL
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  • Gene Information
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Description : Antigen standard for Fas-activated serine/threonine kinase (FASTK), transcript variant 4 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source : HEK 293 cells
Species : Human
Components : This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size : 0.1 mg
Storage Instruction : Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications : ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name : FASTK Fas-activated serine/threonine kinase [ Homo sapiens ]
Official Symbol : FASTK
Synonyms : FASTK; Fas-activated serine/threonine kinase; fas-activated serine/threonine kinase; FAST; FAST kinase;
Gene ID : 10922
mRNA Refseq : NM_033015
Protein Refseq : NP_148936
MIM : 606965
UniProt ID : Q14296
Chromosome Location : 7q35
Function : ATP binding; Fas-activated serine/threonine kinase activity; nucleotide binding; protein binding; protein kinase activity; protein serine/threonine kinase activity; transferase activity;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (5)

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How is FASTK involved in mitochondrial respiration? 11/10/2022

FASTK is a key protein regulating mitochondrial respiration, and knockdown of FASTK leads to mitochondrial respiratory dysfunction and apoptosis.

What is the relationship between HBc protein and FASTK protein? 08/13/2022

HBc proteins downregulate FASTK transcription and expression by repressing promoter activity.

How is FASTK related to the RNA-binding protein TIA1? 08/12/2022

FASTK interacts with the RNA-binding protein TIA1.

Where is the FASTK protein localized? 06/18/2021

FASTK protein is localized to mitochondria.

What is the biological function of FASTK? 03/13/2021

FASTK functions in protein amino acid phosphorylation, apoptosis, signal transduction and extracellular signaling to guide apoptosis.

Customer Reviews (2)

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Reviews
03/01/2021

    Protein products are put into experiments with good effect and reliable experimental results.

    12/04/2020

      The technical staff evaluated my experimental process and results and made many constructive comments.

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