"FAM119A" Related Products

Recombinant Human FAM119A 293 Cell Lysate

Cat.No.: FAM119A-6444HCL
Description: Antigen standard for family with sequence similarity 119, member A (FAM119A), transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: METTL21A?methyltransferase like 21A [?Homo sapiens?(human) ]
Official Symbol: FAM119A
Synonyms: METTL21A; FAM119A; HCA557b; HSPA-KMT; methyltransferase like 21A; protein N-lysine methyltransferase METTL21A; HSPA lysine methyltransferase; methyltransferase-like protein 21A; protein-lysine methyltransferase METTL21A; family with sequence similarity 119, member A; hepatocellular carcinoma-associated antigen 557b; heat shock protein 70kDa lysine (K) methyltransferase; NP_001120867.1; EC 2.1.1.-; NP_660323.3
Gene ID: 151194
mRNA Refseq: NM_145280
Protein Refseq: NP_660323
MIM: 615257
UniProt ID: Q8WXB1
Chromosome Location: 2q33.3
Function: ATPase binding; Hsp70 protein binding; protein binding; protein-lysine N-methyltransferase activity

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