"EZR" Related Products

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Recombinant Human EZR 293 Cell Lysate

Cat.No.: EZR-6486HCL
Description: Antigen standard for ezrin (EZR), transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: EZR ezrin [ Homo sapiens ]
Official Symbol: EZR
Synonyms: EZR; ezrin; VIL2, villin 2 (ezrin); cytovillin 2; p81; villin-2; villin 2 (ezrin); CVL; CVIL; VIL2; MGC1584; FLJ26216; DKFZp762H157;
Gene ID: 7430
mRNA Refseq: NM_001111077
Protein Refseq: NP_001104547
MIM: 123900
UniProt ID: P15311
Chromosome Location: 6q25.3
Pathway: Axon guidance, organism-specific biosystem; Developmental Biology, organism-specific biosystem; FAS (CD95) signaling pathway, organism-specific biosystem; Gastric acid secretion, organism-specific biosystem; Gastric acid secretion, conserved biosystem; L1CAM interactions, organism-specific biosystem; LKB1 signaling events, organism-specific biosystem;
Function: actin filament binding; cell adhesion molecule binding; cytoskeletal protein binding; protein binding;

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