"ERI1" Related Products


Recombinant Human ERI1 293 Cell Lysate

Cat.No.: ERI1-6554HCL
Description: Antigen standard for exoribonuclease 1 (ERI1) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: ERI1 exoribonuclease 1 [ Homo sapiens ]
Official Symbol: ERI1
Synonyms: ERI1; exoribonuclease 1; THEX1, three prime histone mRNA exonuclease 1; 3-5 exoribonuclease 1; 3HEXO; enhanced RNAi three prime mRNA exonuclease homolog 1 (C.elegans); Eri-1 homolog; protein 3hExo; 3 exoribonuclease; 3-5 exonuclease ERI1; histone mRNA 3-exonuclease 1; three prime mRNA exonuclease 1; three prime histone mRNA exonuclease 1; histone mRNA 3 end-specific exonuclease; histone mRNA 3-end-specific exoribonuclease; enhanced RNAi three prime mRNA exonuclease homolog 1; HEXO; THEX1; MGC35395;
Gene ID: 90459
mRNA Refseq: NM_153332
Protein Refseq: NP_699163
MIM: 608739
UniProt ID: Q8IV48
Chromosome Location: 8p23.1
Function: 3-5 exonuclease activity; histone pre-mRNA stem-loop binding; hydrolase activity; metal ion binding; rRNA binding; ribosome binding;

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