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Recombinant Human ERG 293 Cell Lysate

Cat.No.: ERG-6560HCL
Description: Antigen standard for v-ets erythroblastosis virus E26 oncogene homolog (avian) (ERG), transcript variant 2 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: ERG v-ets erythroblastosis virus E26 oncogene homolog (avian) [ Homo sapiens ]
Official Symbol: ERG
Synonyms: ERG; v-ets erythroblastosis virus E26 oncogene homolog (avian); v ets avian erythroblastosis virus E26 oncogene related , v ets erythroblastosis virus E26 oncogene like (avian); transcriptional regulator ERG; erg 3; p55; TMPRSS2 ERG prostate cancer specific; transcriptional regulator ERG (transforming protein ERG); v ets avian erythroblastosis virus E26 oncogene related; v ets erythroblastosis virus E26 oncogene like; ets-related; TMPRSS2/ERG fusion; v-ets erythroblastosis virus E26 oncogene like; v-ets avian erythroblastosis virus E26 oncogene related; erg-3;
Gene ID: 2078
mRNA Refseq: NM_004449
Protein Refseq: NP_004440
MIM: 165080
UniProt ID: P11308
Chromosome Location: 21q22.3
Pathway: Transcriptional misregulation in cancer, organism-specific biosystem; Transcriptional misregulation in cancer, conserved biosystem;
Function: DNA binding; protein binding; sequence-specific DNA binding; sequence-specific DNA binding transcription factor activity; signal transducer activity;

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