Recombinant Human ENPP1 293 Cell Lysate

• Cat.No.: ENPP1-6595HCL

Specification

• Description: Antigen standard for ectonucleotide pyrophosphatase/phosphodiesterase 1 (ENPP1) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: ENPP1 ectonucleotide pyrophosphatase/phosphodiesterase 1 [ Homo sapiens ]
• Official Symbol: ENPP1
• Synonyms: ENPP1; ectonucleotide pyrophosphatase/phosphodiesterase 1; M6S1, NPPS, PDNP1; ectonucleotide pyrophosphatase/phosphodiesterase family member 1; PC 1; PCA1; E-NPP 1; Ly-41 antigen; alkaline phosphodiesterase 1; plasma-cell membrane glycoprotein 1; plasma-cell membrane glycoprotein PC-1; membrane component chromosome 6 surface marker 1; phosphodiesterase I/nucleotide pyrophosphatase 1; membrane component, chromosome 6, surface marker 1; M6S1; NPP1; NPPS; PC-1; ARHR2; PDNP1
• Gene ID: 5167
• mRNA Refseq: NM_006208
• Protein Refseq: NP_006199
• MIM: 173335
• UniProt ID: P22413
• Chromosome Location: 6q22-q23
• Pathway: Endochondral Ossification, organism-specific biosystem; Insulin Signaling, organism-specific biosystem; Metabolic pathways, organism-specific biosystem; Metabolism, organism-specific biosystem; Metabolism of vitamins and cofactors, organism-specific biosystem; Metabolism of water-soluble vitamins and cofactors, organism-specific biosystem; Nicotinate and nicotinamide metabolism, organism-specific biosystem
• Function: 3-phosphoadenosine 5-phosphosulfate binding; ATP binding; NADH pyrophosphatase activity; hydrolase activity; insulin receptor binding; metal ion binding; nucleic acid binding; nucleoside-triphosphate diphosphatase activity; nucleotide diphosphatase activity; nucleotide diphosphatase activity; phosphodiesterase I activity; polysaccharide binding; protein binding; protein homodimerization activity; scavenger receptor activity